Can I read Nerve Growth Factor-stimulated Mitogen-activated Protein Kinase Activity Is Not Necessary for Neurite Outgrowth of Chick Dorsal Root Ganglion Sensory and Sympathetic Neurons on EtoBox?

Nerve Growth Factor-stimulated Mitogen-activated Protein Kinase Activity Is Not Necessary for Neurite Outgrowth of Chick Dorsal Root Ganglion Sensory and Sympathetic Neurons by Franz Josef Klinz; Peter Wolff; Rolf Heumann is a Neuroscience article available to read on EtoBox.

What is Nerve Growth Factor-stimulated Mitogen-activated Protein Kinase Activity Is Not Necessary for Neurite Outgrowth of Chick Dorsal Root Ganglion Sensory and Sympathetic Neurons about?

Nerve growth factor (NGF)-stimulated neurite outgrowth in the rat PC12 tumor cell line recently has been shown to depend on the activation of the mitogen-activated protein (MAP) kinase kinase 1 (MEK1) (Pang et al.: J Biol Chem 270:13585-13588,1995). In this study we have analyzed whether or not function of the MAP kinase pathway is necessary for NGFstimulated neurite outgrowth in two subtypes of primary neurons derived from the embryonic chick peripheral nervous system (PNS). Treatment of p2lras-dependent dorsal root ganglion (DRG) sensory neurons (E9) with the MEKl inhibitor PD98059 at concentrations up to 100 pM did not prevent NGFstimulated neurite outgrowth. At this concentration NGF-stimulated tyrosine phosphorylation of MAP kinase p42 as well as MAP kinase activity both were decreased by approximately 80%. Essentially the same results were obtained with p2lras-independent sympathetic neurons (E12). We conclude that, in contrast to the PC12 tumor cell line, NGF-stimulated MAP kinase activity is not necessary for neurite outgrowth of DRG sensory and sympathetic neurons derived from the chick PNS.

Who reads Nerve Growth Factor-stimulated Mitogen-activated Protein Kinase Activity Is Not Necessary for Neurite Outgrowth of Chick Dorsal Root Ganglion Sensory and Sympathetic Neurons?

Author

Franz Josef Klinz; Peter Wolff; Rolf Heumann

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)