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An efficient 2-keto-L-gulonic acid whole-cell biotransformation system built on the characterization of L-sorbose dehydrogenase by Fan Li; Cai-Yun Wang; Meng-Yue Zhang; Yi-Xuan Zhang is a Chemistry article available to read on EtoBox.

What is An efficient 2-keto-L-gulonic acid whole-cell biotransformation system built on the characterization of L-sorbose dehydrogenase about?

There are five L-sorbose dehydrogenases (SDH\_2764, SDH\_2744, SDH\_0718, SDH\_1927, SDH\_0337) in Ketogulonicigenium vulgare SPU B805 and three L-sorbose dehydrogenases (SDH\_02251, SDH\_02271, SDH\_P100164) in K. robustum SPU\_B003, which play essential roles in the classical two-step fermentation for 2-keto-L-gulonic acid (2-KGA, the precursor of vitamin C) synthesis. In this work, the above eight SDH enzymes were cloned and purified, and their optimal pH values and reaction temperatures, kinetic properties (K m and K cat ), thermal stabilities, substrate spectra, and effects of metal ions were determined. Eight SDH-harboring recombinant E.coli strains were verified to be capable to catalyze L-sorbose to 2-KGA. Additionally, the molecular docking results showed that the product 2-KGA could inhibit SDH activity by competing for the same hydrogen bonding sites with substrate L-sorbose. Finally, a novel and efficient whole-cell recycling catalysis system to overcome the product inhibition was proposed, the yield of 2-KGA reached up to 80.12 g/L with the productivity of 6.68 g/L/h. The results provide a promising prospect for selection and modification sorbose dehydrogenase for cons

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Author

Fan Li; Cai-Yun Wang; Meng-Yue Zhang; Yi-Xuan Zhang

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Chemistry (Physical Sciences)