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This study analyzed the in vitro culture characteristics and growth requirements of the Baby Hamster Kidney-21 (BHK-21) cell line. BHK-21 cells multiply rapidly in the first 48 hours to form a complete monolayer within 72 hours, followed by a decline phase. Fetal calf serum has a growth stimulating effect, with 5-10% serum being satisfactory for cell line maintenance. Trypsin (0.25%) with neutralization by fetal calf serum (5-10%) was found to be effective for cell harvesting. For cryopreservation, 10% Dime

Author

Novena Dp

Language

EN