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Calculating Theoretical Plates in HPLC by RajaRamanD is a document available to read on EtoBox.

The document discusses four common methods for calculating the number of theoretical plates (N) in chromatography: 1) tangent line method, 2) half peak height method, 3) area height method, and 4) EMG (exponentially modified Gaussian) method. It finds that the different methods can produce varying values of N, especially for peaks with significant distortion or tailing. While a method judging broader peaks more severely is generally more practical, it is best to keep using a consistent method for comparison

Author

RajaRamanD

Language

EN