Can I read An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples on EtoBox?

An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples by Ramos-Sono, Daniel ;Laureano, Raúl ;Rueda, Daniel ;Gilman, Robert H. ;La Rosa, Adolfo ;Ruiz, Jesús ;León, Raúl ;Sheen, Patricia ;Zimic, Mirko ;Neyrolles, Olivier (editor) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples about?

Tuberculosis (TB) is a major global public health problem with high mortality and morbidity. In low-middle income countries (LMIC) a large number of respiratory symptomatic cases that require TB screening per year demands more accurate, fast and affordable testing for TB diagnostics. Sputum smear is the initial screening test in LMICs, however, its sensitivity is limited in patients with low sputum bacilli load. The same limitation is observed in the currently available molecular tests. We designed, standardized and evaluated an electrochemical biosensor that detects the highly specific DNA insertion element 6110 (IS6110). A PCR amplified DNA product is hybridized on the surface of the working electrode built on FTO-Glass with immobilized specific DNA probes, after which cyclic voltammetry is performed with an Ag/AgCl reference electrode and a platinum counter electrode. The response of the sensor was measured by the ratio (cathodic peak current of the hybridized sensor) / (cathodic peak current of the non-hybridized sensor). We tested the biosensor, using positive hybridization control sequences, genomic DNA extracted from M. tuberculosis strains and sputum of TB patients, and ext

Who reads An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples?

Author

Ramos-Sono, Daniel ;Laureano, Raúl ;Rueda, Daniel ;Gilman, Robert H. ;La Rosa, Adolfo ;Ruiz, Jesús ;León, Raúl ;Sheen, Patricia ;Zimic, Mirko ;Neyrolles, Olivier (editor)

Publisher

Public Library of Science (PLoS)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)