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What is Endothelial Cell Isolation Protocols about?

This document provides detailed protocols for the isolation of endothelial cells (ECs) from mouse kidney, spleen, and testis tissues, essential for studying their roles in various diseases. The methods utilize a combination of magnetic- and fluorescence-activated cell sorting to achieve high purity and quality for single-cell RNA sequencing. The protocols are designed for reproducibility and efficiency, ensuring optimal conditions for EC purification from adult murine tissues.

Author

Rogerio Faustino

Language

EN