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Degradation of ochratoxins A and B by lipases: A kinetic study unraveled by molecular modeling by Joana Santos; Tarsila Castro; Armando Venâncio; Carla Silva is a Medicine article available to read on EtoBox.

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Mycotoxins are toxic substances produced by fungi and, frequently, different mycotoxins cooccur in food commodities. Ochratoxin A (OTA) and Ochratoxin B (OTB) may co-occur in a variety of foods, like red wines and wheat, presenting a significant risk of population exposure. In this study, we investigated the potential of five lipases (__Candida rugosa__ Lipase, __Candida antarctica__ B Lipase, __Thermomyces lanuginosus__ Lipase, Amano Lipase A from __Aspergillus niger__ (ANL) and Porcine Pancreas Lipase (PPL)) to hydrolyze OTA and OTB into non-hazardous products. Only ANL and PPL degraded both substrates, however, with varying degrees of efficiency. PPL completely degraded OTB (9 h), but only 43% of OTA (25 h). Molecular simulations indicated a high binding energy of OTA to PPL, that can be explained by the impact of the chlorine group, impairing hydrolysis. ANL was able to completely degrade both mycotoxins, OTA in 3 h and OTB in 10 h. The ANL enzyme showed also high specificity to OTA, however, the activity of this enzyme is not affected by chlorine and hydrolyzes OTA faster than OTB. These two enzymes were found to be able to detoxify co-occurring ochratoxins A and B, making iso

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Author

Joana Santos; Tarsila Castro; Armando Venâncio; Carla Silva

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Physical Sciences)