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Brain‐derived neurotrophic factor induces migration of endothelial cells through a TrkB–ERK–integrin α~V~β~3~–FAK cascade by Shinji Matsuda; Tsuyoshi Fujita; Mikihito Kajiya; Katsuhiro Takeda; Hideki Shiba; Hiroyuki Kawaguchi; Hidemi Kurihara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Brain‐derived neurotrophic factor (BDNF) promotes the regeneration of periodontal tissue. Since angiogenesis is important for tissue regeneration, investigating effect of BDNF on endothelial cell function may help to reveal its mechanism, whereby, BDNF promotes periodontal tissue regeneration. In this study, we examined the influence of BDNF on migration in human microvascular endothelial cells (HMVECs), focusing on the effects on extracellular signal‐regulated kinase (ERK), integrin α~V~β~3~, and focal adhesion kinase (FAK). The migration of endothelial cells was assessed with a modified Boyden chamber and a wound healing assay. The expression of integrin α~V~β~3~ and the phosphorylation of ERK and FAK were analyzed by immunoblotting and immunofluorescence microscopy. BDNF (25 ng/ml) induced cell migration. PD98059, an ERK inhibitor, K252a, a specific inhibitor for TrkB, a high affinity receptor of BDNF, and an anti‐integrin α~V~β~3~ antibody suppressed the BDNF‐induced migration. BDNF increased the levels of integrin α~V~β~3~ and phosphorylated ERK1/2 and FAK. The ERK inhibitor and TrkB inhibitor also reduced levels of integrin α~V~β~3~ and phosphorylated FAK. We prop

Who reads Brain‐derived neurotrophic factor induces migration of endothelial cells through a TrkB–ERK–integrin α~V~β~3~–FAK cascade?

Author

Shinji Matsuda; Tsuyoshi Fujita; Mikihito Kajiya; Katsuhiro Takeda; Hideki Shiba; Hiroyuki Kawaguchi; Hidemi Kurihara

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2012

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)