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Direct esterification of the protein amino acids : Gas-Liquid chromatography of N-TFA n-butyl esters by Don Roach; Charles W. Gehrke is a Chemistry article available to read on EtoBox.

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The 'reaction conditions necessary for the "direct esterification" of the protein amino acids to their ~butyl esters are described. All of the amino acids were quantitatively esterified in rc-butanol 3 iV in hydrochloric acid at 100' with the exception of isoleucine, This "direct esterification" method with +z-butanol permits a rapid derivatization and analysis by gas-liquid chromatography of the protein amino acids, thus, one of the major disadvantages of the earlier reported method has been removed. The amino acids were observed to dissolve very slowly'in ut-butanol 6 N in hydrochloric acid even when the samples were subjected to ultrasonic mixing. Fairly rapid dissolution occurred in 1.5 N hydrochloric acid but a longer esterification time was noted. The optimum concentration of hydrochloric acid was found to be 3 N because the amino acids dissolved quickly in this solution with ultrasonic mixing and short esterification times were obtained. The more insoluble amino acids were broken up by ultrasonic mixing, thus increased rates of solution and esterification to the qz-butyl esters were achieved. The effect of temperature over the range of go to 220°, on the rate of esterificati

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Author

Don Roach; Charles W. Gehrke

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1969

Language

EN

Field

Chemistry (Physical Sciences)