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This document describes the development of species-specific polymerase chain reaction (PCR) primers to detect Rhodococcus equi. Random amplification of polymorphic DNA from R. equi and R. rhodochrous reference strains identified a unique 700-base pair fragment specific to R. equi. PCR with these primers produced a product only from R. equi isolates and not other Rhodococcus species or other gram-positive and gram-negative bacteria. The primers allow specific detection of R. equi, an important cause of pneum

Author

Kishor Kumar

Language

EN