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Involvement of caspase‐3 and p38 mitogen‐activated protein kinase in cobalt chloride‐induced apoptosis in PC12 cells by Weiguo Zou; Jiping Zeng; Ming Zhuo; Weijing Xu; Lanying Sun; Jinxing Wang; Xinyuan Liu is a Neuroscience article available to read on EtoBox.

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## Abstract Our previous study showed that cobalt chloride (CoCl~2~) could induce PC12 cell apoptosis and that the CoCl~2~‐treated PC12 cells may serve as a simple in vitro model for the study of the mechanism of hypoxia‐linked neuronal disorders. The aim of this study is to elucidate the mechanism of CoCl~2~‐induced apoptosis in PC12 cells. Caspases are known to be involved in the apoptosis induced by various stimuli in many cell types. To investigate the involvement of caspases in CoCl~2~‐induced apoptosis in PC12 cells, we generated PC12 cells that stably express the viral caspases inhibitor gene p35 and analyzed the effect of p35 on the process of apoptosis induced by CoCl~2~. We also examined the effect of cell‐permeable peptide inhibitors of caspases. The results showed that the baculovirus p35 gene and the general caspases inhibitor Z‐VAD‐FMK significantly block apoptosis induced by CoCl~2~, confirming that caspase is involved in CoCl~2~‐induced apoptosis. Further investigation showed that in this process the caspase‐3‐like activity is increased, as indicated by the cells' ability to cleave the fluorogenic peptide substrate Ac‐Asp‐Glu‐Val‐Asp‐7‐AMC and to degrade the DNA‐rep

Who reads Involvement of caspase‐3 and p38 mitogen‐activated protein kinase in cobalt chloride‐induced apoptosis in PC12 cells?

Author

Weiguo Zou; Jiping Zeng; Ming Zhuo; Weijing Xu; Lanying Sun; Jinxing Wang; Xinyuan Liu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)