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Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye by Axel Landgraf; Bernd Reckmann; Alfred Pingoud is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye about?

PCR primers covalently labeled with biotin and a fluorescent dye allow immobilization and separation of the products which can be quantitatively analyzed subsequently. The procedure we have developed circumvents electrophoretic separation and radioactive labeling. Exact quantitative analysis of reaction products is feasible during the logarithmic phase of amplification when Taq polymerase is not limiting, as it is during the plateau phase of the reaction. With appropriate standardization the procedure can be used for routine diagnostic purposes.

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Author

Axel Landgraf; Bernd Reckmann; Alfred Pingoud

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)