Opening book details…
Can I read High-Affinity Anti-Ganglioside IgG Antibodies Raised in Complex Ganglioside Knockout Mice : Reexamination of GD1a Immunolocalization on EtoBox?
High-Affinity Anti-Ganglioside IgG Antibodies Raised in Complex Ganglioside Knockout Mice : Reexamination of GD1a Immunolocalization by Michael P. T. Lunn; L'Aurelle A. Johnson; Susan E. Fromholt; Saki Itonori; Jian Huang; Alka A. Vyas; James E. K. Hildreth; John W. Griffin; Ronald L. Schnaar; Kazim A. Sheikh is a Neuroscience article available to read on EtoBox.
What is High-Affinity Anti-Ganglioside IgG Antibodies Raised in Complex Ganglioside Knockout Mice : Reexamination of GD1a Immunolocalization about?
**Abstract** : Gangliosides, sialic acid‐bearing glycosphingolipids, are highly enriched in the vertebrate nervous system. Anti‐ganglioside antibodies are associated with various human neuropathies, although the pathogenicity of these antibodies remains unproven. Testing the pathogenic role of anti‐ganglioside antibodies will be facilitated by developing high‐affinity IgG‐class complement‐fixing monoclonal anti‐bodies against major brain gangliosides, a goal that has been difficult to achieve. In this study, mice lacking complex gangliosides were used as immune‐naive hosts to raise anti‐ganglioside antibodies. Wild‐type mice and knockout mice with a disrupted gene for GM2/GD2 synthase (UDP‐__N__‐acetyl‐D‐galactosamine : GM3/GD3 __N__‐acetyl‐D‐glactosaminyltransferase) were immunized with GD1a conjugated to keyhole limpet hemocyanin. The knockout mice produced a vigorous anti‐GD1a IgG response, whereas wildtype littermates failed to do so. Fusion of spleen cells from an immunized knockout mouse with myeloma cells yielded numerous IgG anti‐GD1a antibody‐producing colonies. Ganglioside binding studies revealed two specificity classes ; one colony representing each class was cloned and
Who reads High-Affinity Anti-Ganglioside IgG Antibodies Raised in Complex Ganglioside Knockout Mice : Reexamination of GD1a Immunolocalization?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Michael P. T. Lunn; L'Aurelle A. Johnson; Susan E. Fromholt; Saki Itonori; Jian Huang; Alka A. Vyas; James E. K. Hildreth; John W. Griffin; Ronald L. Schnaar; Kazim A. Sheikh
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2000
- Language
- EN
- Field
- Neuroscience (Life Sciences)