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V~β~‐restricted T cell adherence to endothelial cells: A mechanism for superantigen‐dependent vascular injury by P. A. Brogan; V. Shah; N. Klein; M. J. Dillon is a Medicine article available to read on EtoBox.

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## Abstract ## Objective To investigate the potential for endothelial cells to operate as superantigen‐presenting cells for T cells and the potential for such an interaction to cause endothelial cell activation and injury. ## Methods Class II major histocompatibility complex (MHC)–positive human umbilical vein endothelial cells (HUVECs) were cocultured for 4 hours with purified T cells and the superantigens staphylococcal enterotoxin B (SEB) or toxic shock syndrome toxin 1 (TSST‐1). After staining with fluorescence‐conjugated monoclonal antibodies, flow cytometric analysis was performed on the HUVECs and T cells to examine V~β~‐restricted T cell adherence to the endothelial cell monolayer, V~β~‐restricted T cell activation (CD69 up‐regulation), surface expression of endothelial cell activation markers, and generation of endothelial microparticles (EMPs). ## Results Coculture of purified T cells with class II MHC–positive HUVECs and either TSST‐1 or SEB resulted in V~β~‐restricted CD69 up‐regulation by CD4 and CD8 cells (V~β~2 activation for TSST‐1; V~β~3, V~β~5.1, and V~β~12 activation for SEB). Additionally, there was CD4 and CD8 T cell V~β~‐restricted adherence to the HUVEC monol

Who reads V~β~‐restricted T cell adherence to endothelial cells: A mechanism for superantigen‐dependent vascular injury?

Author

P. A. Brogan; V. Shah; N. Klein; M. J. Dillon

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2004

Language

EN

Field

Medicine (Health Sciences)