Can I read Comparison of RT-PCR with other diagnostic assays for rapid detection of influenza viruses on EtoBox?

Comparison of RT-PCR with other diagnostic assays for rapid detection of influenza viruses by Pregliasco, Fabrizio; Mensi, Carolina; Camorali, Laura; Anselmi, Giovanni is a Medicine article available to read on EtoBox.

What is Comparison of RT-PCR with other diagnostic assays for rapid detection of influenza viruses about?

To compare the effectiveness of reverse transcription-polymerase chain reaction (RT-PCR), shell vial culture and cytospin assay as laboratory techniques for rapid diagnosis of influenza infections, a retrospective study was carried out on 270 aliquots of oropharyngeal swabs collected from October 1993 to March 1996 and already characterized by standard isolation procedures, and a prospective study in which 65 clinical samples taken from patients with influenza-like syndrome between October 1996 and March 1997 were tested. In the retrospective study, using conventional isolation as the gold standard, the sensitivity of RT-PCR and cytospin assay for virus A was 100% (95% confidence interval (CI), 89.1-100) and for virus B it was 100% (95% CI, 56.1-100) compared with 77.5% (95% CI, 61.1-88.6) and 71.4% (95% CI, 30.3-94.9) for shell vial culture. The specificity of all the three assays was 100% (95% CI, 98.0-100) for virus A and 100% (95% CI, 98.2-100) for virus B. In the prospective study the sensitivity of RT-PCR was greater than that of the other tests considered, both rapid and standard. It is suggested that RT-PCR should be employed in combination with conventional culture techniq

Who reads Comparison of RT-PCR with other diagnostic assays for rapid detection of influenza viruses?

Author

Pregliasco, Fabrizio; Mensi, Carolina; Camorali, Laura; Anselmi, Giovanni

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

1998

Language

EN

Field

Medicine (Health Sciences)