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Can I read Knockdown of Repulsive Guidance Molecule a Promotes Polarization of Microglia Into an Anti-inflammatory Phenotype After Oxygen-glucose Deprivation-reoxygenation in Vitro on EtoBox?
Knockdown of Repulsive Guidance Molecule a Promotes Polarization of Microglia Into an Anti-inflammatory Phenotype After Oxygen-glucose Deprivation-reoxygenation in Vitro by Guanru Shen; Hongmei Xiao; Siyuan Huang; Xiaofan Yuan; Zhang Rongrong; Yue Ma; Xinyue Qin is a Neuroscience article available to read on EtoBox.
What is Knockdown of Repulsive Guidance Molecule a Promotes Polarization of Microglia Into an Anti-inflammatory Phenotype After Oxygen-glucose Deprivation-reoxygenation in Vitro about?
Repulsive guidance molecule a (RGMa) is a glycosylphosphatidylinositol-anchored glycoprotein that has been demonstrated to influence neuroinflammatory-related diseases in addition to regulating neuronal differentiation and survival during brain development. However, any function or mechanism of RGMa in the polarization of microglia after ischemic stroke remains unclear. In the current study, RGMa was found to be expressed at reduced levels in microglia after oxygen-glucose deprivation-reoxygenation (OGD/R) in vitro. RGMa overexpression induced HAPI microglia to predominantly polarize to the M1 phenotype, promoting the release of proinflammatory cytokines and knockdown induced the M2 phenotype, promoting the release of anti-inflammatory cytokines. RGMa overexpression also regulated the polarization of HAPI microglia by inhibiting the transportation of peroxisome proliferator-activated receptor γ (PPARγ) from the nucleus to cytoplasm. The opposite effect resulted from RGMa-knockdown and was reversed by the PPARγ antagonist, GW9662. In addition, RGMa-knockdown HAPI microglial conditioned medium improved the survival of oligodendrocytes after OGD/R in vitro. Thus, inhibition of RGMa ma
Who reads Knockdown of Repulsive Guidance Molecule a Promotes Polarization of Microglia Into an Anti-inflammatory Phenotype After Oxygen-glucose Deprivation-reoxygenation in Vitro?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Guanru Shen; Hongmei Xiao; Siyuan Huang; Xiaofan Yuan; Zhang Rongrong; Yue Ma; Xinyue Qin
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Neuroscience (Life Sciences)