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Turbidimetric Assay for Pyocin Activity by youkaho is a document available to read on EtoBox.
This document describes a new turbidimetric method for quantitatively assaying the activity of pyocins, which are bacteriocins (antibacterial proteins) produced by Pseudomonas aeruginosa. The method involves incubating dilutions of a pyocin preparation with a suspension of indicator bacteria sensitive to that pyocin. Bacterial growth is then measured turbidimetrically after incubation. The results are analyzed using probit analysis to determine the pyocin concentration required to inhibit 50% of bacterial g
- Author
- youkaho
- Language
- EN