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Evidence that Protein Kinase C Activities Involved in Regulating Neurite Growth Are Localized to Distal Neurites by Robert B. Campenot; Dwight D. Draker; Donna L. Senger is a Neuroscience article available to read on EtoBox.

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Abstract: Previously, we observed that long‐term treatment of distal nerve fibers of rat sympathetic neurons in compartmented cultures with phorbol 12‐myristate 13‐acetate (PMA) caused a reduction in the rate of neurite elongation by >50%. In the present report we show that protein kinase C (PKC) activity could be measured in extracts of distal neurites by an assay of the Ca^2+^‐dependent phosphorylation of a PKC‐specific octapeptide substrate. We found that local application of 1 μ__M__ PMA for 24 h to distal neurites caused nearly complete down‐regulation of Ca^2+^‐dependent PKC activity measured in this manner. We determined that the inhibition of neurite elongation by PMA was mediated by local mechanisms in the neurites because local application of PMA to center compartments containing cell bodies and proximal neurites did not inhibit the rate of elongation of distal neurites. We then investigated the effects of the recently available PKC inhibitors, calphostin C and chelerythrine, finding that, like PMA, these inhibited the growth of distal neurites when applied locally to them, and had no effect when applied to cell bodies and proximal neurites. However, the inhibition of neu

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Author

Robert B. Campenot; Dwight D. Draker; Donna L. Senger

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1994

Language

EN

Field

Neuroscience (Life Sciences)