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An Imaging Flow Cytometric Method for Measuring Cell Division History and Molecular Symmetry During Mitosis by Andrew Filby; Esperanza Perucha; Huw Summers; Paul Rees; Prabhjoat Chana; Susanne Heck; Graham M. Lord; Derek Davies is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An Imaging Flow Cytometric Method for Measuring Cell Division History and Molecular Symmetry During Mitosis about?

## Abstract Asymmetric cell division is an important mechanism for generating cellular diversity, however, techniques for measuring the distribution of fate‐regulating molecules during mitosis have been hampered by a lack of objectivity, quantitation, and statistical robustness. Here we describe a novel imaging flow cytometric approach that is able to report a cells proliferative history and cell cycle position using dye dilution, pH3, and PI staining to then measure the spatial distribution of fluorescent signals during mitosis using CCD‐derived imagery. Using Jurkat cells, resolution of the fluorescently labeled populations was comparable to traditional PMT based cytometers thus eliminating the need to sort cells with specific division histories for microscopy. Subdividing mitotic stages by morphology allowed us to determine the time spent in each cell cycle phase using mathematical modeling approaches. Furthermore high sample throughput allowed us to collect statistically relevant numbers of cells without the need to use blocking agents that artificially enrich for mitotic events. The fluorescent imagery was used to measure PKCζ protein and EEA‐1+ endosome distribution during di

Who reads An Imaging Flow Cytometric Method for Measuring Cell Division History and Molecular Symmetry During Mitosis?

Author

Andrew Filby; Esperanza Perucha; Huw Summers; Paul Rees; Prabhjoat Chana; Susanne Heck; Graham M. Lord; Derek Davies

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)