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Isolation and characterization of a new human urinary metabolite of diclofenac applying LC–NMR–MS and high-resolution mass analyses by Dele Stülten; Marc Lamshöft; Sebastian Zühlke; Michael Spiteller is a Chemistry article available to read on EtoBox.

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The nonsteroidal anti-inflammatory drug diclofenac is widely used. Diclofenac is extensively metabolized to several hydroxylated derivatives and their conjugates. The lactam-dehydrate of 4 -hydroxy diclofenac (4 -OHDD) has now been detected as a new urinary metabolite of diclofenac. Isolation was successfully performed using preparative HPLC in three different steps using water, methanol, and acetonitrile, respectively. The structural characterization of 4 -OHDD was achieved by LC-NMR-MS. In addition, specific mass fragmentation pattern could be obtained using LC-high-resolution MS with both positive and negative electrospray ionization.

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Author

Dele Stülten; Marc Lamshöft; Sebastian Zühlke; Michael Spiteller

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)