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Importance of Magnesium in PCR by Janak Awasthi is a document available to read on EtoBox.
What is Importance of Magnesium in PCR about?
PCR requires magnesium and thermostable polymerase in addition to nucleotides and buffer because magnesium is necessary for polymerase to function and replicate DNA, while thermostable polymerase like Taq can withstand the high temperatures used to separate DNA strands, unlike human polymerase.
- Author
- Janak Awasthi
- Language
- EN