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A Polydiacetylene Microchip Based on a Biotin–Streptavidin Interaction for the Diagnosis of Pathogen Infections by Yun Kyung Jung; Tae Won Kim; Cheulhee Jung; Dae-Yeon Cho; Hyun Gyu Park is a Engineering article available to read on EtoBox.

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## Abstract A micropatterned polydiacetylene (PDA) chip, utilizing the unique fluorogenic property of PDA and a specific biotin–streptavidin (STA) interaction, is constructed to detect pathogen infections. To construct the PDA chip, biotin‐modified diacetylene liposomes are immobilized on aldehyde glass and conjugated with STA, followed by UV irradiation to polymerize the STA‐functionalized diacetylene liposomes. Genomic DNA of a model pathogen, __Chlamydia trachomatis__, is isolated from human samples and biotin‐labeled target DNA is obtained through PCR amplification using biotin‐11‐dUTP. Owing to the stimulus caused by the biotin–STA interaction, the biotinylated DNA induces an intense fluorescence signal on the immobilized PDA. By using this strategy, it is possible to diagnose __Chlamydia__ infections by applying DNA samples from several nonhealthy humans to a single PDA chip. The results of this study serve as the basis for a new strategy for fluorogenic PDA microarray‐based diagnosis of pathogen infections.

Who reads A Polydiacetylene Microchip Based on a Biotin–Streptavidin Interaction for the Diagnosis of Pathogen Infections?

Author

Yun Kyung Jung; Tae Won Kim; Cheulhee Jung; Dae-Yeon Cho; Hyun Gyu Park

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Publons; Wiley (ISSN 1613-6810)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)