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Long‐Term Effects of Ciliary Neurotrophic Factor on the Survival of Vasopressin Magnocellular Neurones in the Rat Supraoptic Nucleus In Vitro by M. Rusnak; S. B. House; H. Gainer is a Neuroscience article available to read on EtoBox.
What is Long‐Term Effects of Ciliary Neurotrophic Factor on the Survival of Vasopressin Magnocellular Neurones in the Rat Supraoptic Nucleus In Vitro about?
## Abstract The use of hypothalamic organotypic cultures for the long‐term study of mechanisms in magnocellular neurones (MCNs) of the hypothalamic‐neurohypophysial system has been limited by the relatively poor maintenance of the vasopressin MCNs __in vitro__. Recent studies have shown that addition of ciliary neurotrophic factor (CNTF) to the media significantly reduced the apoptosis of both oxytocin and vasopressin MCNs. Here, we studied various temporal factors in the CNTF treatment that can influence the efficacy of MCN survival. Immunohistochemistry was used to identify and count surviving vasopressin and oxytocin MCNs in the supraoptic nucleus (SON) in hypothalamic slices cultured in the presence of CNTF (10 ng/ml media) for various time intervals, and __in situ__ hybridization for vasopressin mRNA was used to evaluate the vasopressin mRNA gene expression in the SON under the same conditions. The presence of CNTF in the medium for 10 days produced a maximal increase in the survival of vasopressin MCNs (by 11‐fold) and in the survival of oxytocin‐MCNs (by approximately four‐fold) over controls. These effects persisted for an additional 7–10 days even in the absence of CNTF. T
Who reads Long‐Term Effects of Ciliary Neurotrophic Factor on the Survival of Vasopressin Magnocellular Neurones in the Rat Supraoptic Nucleus In Vitro?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- M. Rusnak; S. B. House; H. Gainer
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-8194)
- Published
- 2003
- Language
- EN
- Field
- Neuroscience (Life Sciences)