Can I read Mechanical Continuity and Reversible Chromosome Disassembly Within Intact Genomes Removed from Living Cells on EtoBox?

Mechanical Continuity and Reversible Chromosome Disassembly Within Intact Genomes Removed from Living Cells by Andrew J. Maniotis; Krzysztof Bojanowski; Donald E. Ingber is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Mechanical Continuity and Reversible Chromosome Disassembly Within Intact Genomes Removed from Living Cells about?

Chromatin is thought to be structurally discontinuous because it is packaged into morphologically distinct chromosomes that appear physically isolated from one another in metaphase preparations used for cytogenetic studies. However, analysis of chromosome positioning and movement suggest that different chromosomes often behave as if they were physically connected in interphase as well as mitosis. To address this paradox directly, we used a microsurgical technique to physically remove nucleoplasm or chromosomes from living cells under isotonic conditions. Using this approach, we found that pulling a single nucleolus or chromosome out from interphase or mitotic cells resulted in sequential removal of the remaining nucleoli and chromosomes, interconnected by a continuous elastic thread. Enzymatic treatments of interphase nucleoplasm and chromosome chains held under tension revealed that mechanical continuity within the chromatin was mediated by elements sensitive to DNase or micrococcal nuclease, but not RNases, formamide at high temperature, or proteases. In contrast, mechanical coupling between mitotic chromosomes and the surrounding cytoplasm appeared to be mediated by gelsolin-sen

Who reads Mechanical Continuity and Reversible Chromosome Disassembly Within Intact Genomes Removed from Living Cells?

Author

Andrew J. Maniotis; Krzysztof Bojanowski; Donald E. Ingber

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)