Opening book details…
Can I read Molecularly Imprinted Polymer Nanogel-based Fluorescence Sensing of Pork Contamination in Halal Meat Extracts on EtoBox?
Molecularly Imprinted Polymer Nanogel-based Fluorescence Sensing of Pork Contamination in Halal Meat Extracts by Cheubong, Chehasan; Takano, Eri; Kitayama, Yukiya; Sunayama, Hirobumi; Minamoto, Kazuaki; Takeuchi, Ryota; Furutani, Shunsuke; Takeuchi, Toshifumi is a Engineering article available to read on EtoBox.
What is Molecularly Imprinted Polymer Nanogel-based Fluorescence Sensing of Pork Contamination in Halal Meat Extracts about?
Pork contamination is a serious concern for the global halal food market because many manufacturers commonly use pork instead of beef to reduce production costs. In this study, a highly sensitive fluorescent molecularly imprinted polymer nanogel (F-MIP-NG)-based sensor was developed for rapid porcine serum albumin (PSA) detection to investigate pork contamination in halal meat extracts. F-MIP-NGs were prepared via molecular imprinting and conjugation with ATTO 647N as the fluorescent reporter molecule for the post-imprinting modification (PIM) and then immobilized on gold-coated sensor chips. For achieving rapid and easy measurement, the fluorescence response was measured using a custom-made liquid handling robot equipped with a fluorescence microscope. The fluorescence response increased with increasing PSA concentration. Under optimal conditions, the F-MIP-NG-based sensors exhibited high sensitivity, a detection limit of 40 pM, a linear range of 0.25-5 nM, and excellent affinity and selectivity towards PSA, compared to potentially interfering proteins. Moreover, it was more efficient to detect beef contamination in 1 wt% pork contamination compared to the real-time polymerase cha
Who reads Molecularly Imprinted Polymer Nanogel-based Fluorescence Sensing of Pork Contamination in Halal Meat Extracts?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Cheubong, Chehasan; Takano, Eri; Kitayama, Yukiya; Sunayama, Hirobumi; Minamoto, Kazuaki; Takeuchi, Ryota; Furutani, Shunsuke; Takeuchi, Toshifumi
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2020
- Language
- EN
- Field
- Engineering (Physical Sciences)