Opening book details…
Can I read Molecular Characterisation of Bacterial endophytes from the Medicinal Plant Diplorhynchus condylocarpon on EtoBox?
Molecular Characterisation of Bacterial endophytes from the Medicinal Plant Diplorhynchus condylocarpon by Ntobeko Dube; Idah Sithole-Niang is a Medicine article available to read on EtoBox.
What is Molecular Characterisation of Bacterial endophytes from the Medicinal Plant Diplorhynchus condylocarpon about?
Endophytes are microbes found within live plant tissues and are known to be reservoirs of bioactive compounds that promote plant growth and antimicrobial activity. This makes them important, but are understudied. In this study endophytes from Diplorhynchus condylocarpon plant were characterized at molecular level and their potential to produce bioactive compounds with antimicrobial activity was explored. DNA extraction was carried out on twenty-four endophytes isolated from leaves and twigs of Diplorhynchus condylocarpon. To confirm the nature of these endophytes at kingdom level the 16S rRNA gene was amplified using the Polymerase chain reaction and all indicated to be prokaryotes in nature. Nine 16S rRNA amplicons out of twenty-four were sequenced and species were identified as Izhakiella capsodis, Escherichia fergusonii, Shigella dysenteriae, Xernorhabdus szentirmaii, Providencia rettgeri, Dickeya zeae and Escherichia albertii. Agar well diffusion was used to test for antimicrobial activity of twenty-four endophytes. All twenty-four endophytes exhibited antimicrobial activity against E. coli and S. aureus, with wide range of zones ranging from 19 to 65 mm. Using KEGG database it
Who reads Molecular Characterisation of Bacterial endophytes from the Medicinal Plant Diplorhynchus condylocarpon?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Ntobeko Dube; Idah Sithole-Niang
- Publisher
- Acta Scientific Publications Pvt. Ltd.
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)