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Luminal and Mucosal-associated Intestinal Microbiota in Patients with Diarrhea-predominant Irritable Bowel Syndrome by Ian M Carroll; Young-Hyo Chang; Jiwon Park; R Balfour Sartor; Yehuda Ringel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Luminal and Mucosal-associated Intestinal Microbiota in Patients with Diarrhea-predominant Irritable Bowel Syndrome about?
## Background Recent studies have suggested a role for an altered intestinal microbiota in the pathophysiology of irritable bowel syndrome (IBS). However, no consensus has been reached regarding the association between specific enteric bacterial groups and IBS. The aim of this study was to investigate the fecal and mucosal-associated microbiota using two independent techniques in intestinal samples from diarrhea-predominant IBS (D-IBS) and healthy controls. ## Methods Fecal and colonic mucosal biopsy samples were obtained from 10 D-IBS patients and 10 healthy controls. Colonic tissue was collected during a un-sedated un-prepped flexible sigmoidoscopy. Fecal and tissue samples were processed immediately upon collection for culture under aerobic and anaerobic conditions or frozen for further molecular analysis. DNA was extracted from all frozen samples and used to enumerate specific bacterial groups using quantitative real-time PCR (qPCR). ## Results Culture analysis of intestinal samples demonstrated a significant reduction in the concentration of aerobic bacteria in fecal samples from D-IBS patients when compared to healthy controls (1.4 × 10^7^ vs. 8.4 × 10^8^ CFUs/g feces, __P__
Who reads Luminal and Mucosal-associated Intestinal Microbiota in Patients with Diarrhea-predominant Irritable Bowel Syndrome?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ian M Carroll; Young-Hyo Chang; Jiwon Park; R Balfour Sartor; Yehuda Ringel
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2009-; Springer Science and Business Media LLC (ISSN 1757-4749)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)