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Establishment and growth characteristics of a cell suspension culture of Marchantia polymorpha L. with high chlorophyll content by Yoshimoto Ohta; Kenji Katoh; Keiko Miyake is a Agricultural and Biological Sciences article available to read on EtoBox.

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A green-pigmented cell suspension culture of Marchantia polymorpha was established using the medium of Murashige and Skoog with addition of organic acids of the tricarboxylic-acid cycle, vitamins and sugars plus sugar alcohols, exclusion of kinetin, and replacement of sucrose with glucose. In continuous light, the cells grew exponentially for ca. 10 days; in the dark, they grew only to a slight extent. The light-grown cells contained well-developed chloroplasts, and chlorophyll content reached almost twice that of the intact gametophyte.

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Author

Yoshimoto Ohta; Kenji Katoh; Keiko Miyake

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1977

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)