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Lactate isotopomer analysis by ^1^H NMR spectroscopy: Consideration of long‐range nuclear spin–spin interactions by Steven G. Lloyd; Huadong Zeng; PeiPei Wang; John C. Chatham is a Medicine article available to read on EtoBox.

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## Abstract Lactate is a key metabolite and its rates of cellular uptake and release, its production rates from glucose and glycogen, and its interconversion rate with pyruvate are important determinants of cellular energy production. If lactate precursors such as pyruvate and glucose are labeled appropriately with ^13^C, ^1^H NMR spectroscopy provides a means of quantifying lactate production from each source and allows measurement of all these rates within a single experiment. However, due to the multiplicities of the resonance lines (from nuclear spin–spin couplings) in lactate ^13^C isotopomers, the ^1^H NMR spectra were found to be more complex than expected, requiring determination of all spin–spin interactions in this anion. All such values were determined for lactate and its precursor pyruvate. The method was then applied to simultaneously measure the rates of exogenous lactate uptake and rates of release of glucose‐, glycogen‐, and pyruvate‐derived lactate in the isolated perfused rat heart. Magn Reson Med 51:1279–1282, 2004. © 2004 Wiley‐Liss, Inc.

Who reads Lactate isotopomer analysis by ^1^H NMR spectroscopy: Consideration of long‐range nuclear spin–spin interactions?

Author

Steven G. Lloyd; Huadong Zeng; PeiPei Wang; John C. Chatham

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0740-3194)

Published

2004

Language

EN

Field

Medicine (Health Sciences)