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Imaging of Individual Biopolymers and Supramolecular Assemblies Using Noncontact Atomic Force Microscopy by Theresa M. McIntire; David A. Brant is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Imaging of Individual Biopolymers and Supramolecular Assemblies Using Noncontact Atomic Force Microscopy about?

A variety of biopolymers is imaged using noncontact atomic force microscopy. Samples are prepared by aerosol spray deposition of aqueous solutions on freshly cleaved mica followed by air drying. The distributions of contour lengths and chain or fibril thicknesses normal to the mica substrate can be measured for individual polymer molecules or molecular assemblies. In many cases it is possible to conclude that the structures imaged and quantitatively analyzed are representative of those present in solution and not artifacts of the deposition/dessication process. Imaging of linear and cyclic triple helices of the polysaccharide scleroglucan is demonstrated. Measurements of the triple helix thickness normal to the mica surface are analyzed, and successful measurements of the molecular weight distribution and mean molar mass are described. It is demonstrated that the extent of chain association in the polysaccharide xanthan can be modulated by the addition of low molecular weight salts. The contour length and chain thickness distributions in a xanthan fraction are presented. Increases in the extent of chain association with increasing polymer concentration are documented for the gellin

Who reads Imaging of Individual Biopolymers and Supramolecular Assemblies Using Noncontact Atomic Force Microscopy?

Author

Theresa M. McIntire; David A. Brant

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)