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Can I read Lipolytic activity from Halobacteria : Screening and hydrolase production on EtoBox?
Lipolytic activity from Halobacteria : Screening and hydrolase production by Tej Bhatnagar; Saad Boutaiba; Hocine Hacene; Jean-Luc Cayol; Marie-Laure Fardeau; Bernard Ollivier; Jacques C Baratti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Lipolytic activity from Halobacteria : Screening and hydrolase production about?
Strains of Halobacteria from an Algerian culture collection were screened for their lipolytic activity against p-nitrophenyl butyrate (PNPB) and p-nitrophenyl palmitate (PNPP). Most strains were active on both esters and 12% hydrolyzed olive oil. A strain identified as Natronococcus sp. was further studied. It grew optimally at 3.5 M NaCl, pH 8 and 40 degrees C. An increase in temperature shifted the optimum salt concentration range for growth from a wider range of 2-4 M, obtained at 25-30 degrees C, to a narrower range of 3.5-4 M, obtained at 35-40 degrees C. At 45 degrees C the optimum salt concentration was 2 M. These results show a clear correlation between salt and temperature requirement. The optimum conditions for the production of hydrolytic activity during growth were: 3.5 M NaCl and pH 8 for PNPB hydrolytic activity and 4 M NaCl and pH 7.5 for PNPP hydrolytic activity; both at 40 degrees C. The clear supernatant of cells grown at 4 M NaCl showed olive oil hydrolysis activity (in presence of 4 M NaCl) demonstrating the occurrence of a lipase activity in this strain. To our knowledge, this is the first report of a lipase activity at such high salt concentration.
Who reads Lipolytic activity from Halobacteria : Screening and hydrolase production?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Tej Bhatnagar; Saad Boutaiba; Hocine Hacene; Jean-Luc Cayol; Marie-Laure Fardeau; Bernard Ollivier; Jacques C Baratti
- Publisher
- John Wiley and Sons; Oxford University Press; Oxford University Press (OUP); Elsevier BV (ISSN 0378-1097)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)