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Can I read Development of Smart Anti-glycan Reagents Using Immunized Lampreys on EtoBox?
Development of Smart Anti-glycan Reagents Using Immunized Lampreys by McKitrick, Tanya R.; Goth, Christoffer K.; Rosenberg, Charles S.; Nakahara, Hirotomo; Heimburg-Molinaro, Jamie; McQuillan, Alyssa M.; Falco, Rosalia; Rivers, Nicholas J.; Herrin, Brantley R.; Cooper, Max D.; Cummings, Richard D. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Development of Smart Anti-glycan Reagents Using Immunized Lampreys about?
Studies on the expression of cellular glycans are limited by a lack of sensitive tools that can discriminate specific structural features. Here we describe the development of a robust platform using immunized lampreys (__Petromyzon marinus__), which secrete variable lymphocyte receptors called VLRBs as antibodies, for generating libraries of anti-glycan reagents. We identified a wide variety of glycan-specific VLRBs detectable in lamprey plasma after immunization with whole fixed cells, tissue homogenates, and human milk. The cDNAs from lamprey lymphocytes were cloned into yeast surface display (YSD) libraries for enrichment by multiple methods. We generated VLRB-Ig chimeras, termed smart anti-glycan reagents (SAGRs), whose specificities were defined by microarray analysis and immunohistochemistry. 15 VLRB antibodies were discovered that discriminated between linkages, functional groups and unique presentations of the terminal glycan motif. The development of SAGRs will enhance future studies on glycan expression by providing sequenced, defined antibodies for a variety of research applications.
Who reads Development of Smart Anti-glycan Reagents Using Immunized Lampreys?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- McKitrick, Tanya R.; Goth, Christoffer K.; Rosenberg, Charles S.; Nakahara, Hirotomo; Heimburg-Molinaro, Jamie; McQuillan, Alyssa M.; Falco, Rosalia; Rivers, Nicholas J.; Herrin, Brantley R.; Cooper, Max D.; Cummings, Richard D.
- Publisher
- Springer Science and Business Media LLC; [London]: Springer Nature, 2018-; Society for Mining, Metallurgy and Exploration Inc. (ISSN 2399-3642)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)