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What is Bilirubin Testing Procedure and Values about?

This document provides instructions for using a colorimetric method to measure total and direct bilirubin levels in serum or plasma samples. The method uses a reaction between bilirubin and diazonium salt of sulphanilic acid to form azobilirubin, which is measured spectrophotometrically. In the presence of DMSO, total bilirubin reacts, while only conjugated bilirubin reacts in its absence. Normal bilirubin levels are provided. The reagents, procedure, calculations, reference, and quality control are describ

Author

Munawwar Awa

Language

EN