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A physical map of the permuted genome of bacteriophage T1 by Neil Ramsay; Donald A. Ritchie is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A physical map of the permuted genome of bacteriophage T1 about?

A restriction map has been constructed for the DNA of coliphage T1 which locates the cleavage sites of the restriction endonucleases, BglI (6 cuts), BglII (16 cuts), EcoRI (2 cuts), HindIII (2 cuts) and PstI (2 cuts). Digestions with BglI and BglII reveal fragments which are present in sub-molar quantities. Two methods, one using the selective removal of molecular ends with exonuclease III and the other involving the comparison of digestion patterns of concatemeric and virion DNA, have shown that the submolar fragments are at or close to the ends of the molecules. Digestions with BglI show that one terminal fragment has a very precise molecular weight whereas all the others are of heterogenous molecular weight. These results are consistent with the model for DNA packaging in which maturation is initiated at a precise site on a concatemeric precursor and proceeds by the encapsidation of up to four successive 'headfuls' of 1.065 genome equivalents (MacHattie and Gill 1977).

Who reads A physical map of the permuted genome of bacteriophage T1?

Author

Neil Ramsay; Donald A. Ritchie

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1980

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)