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Production of Monoclonal Antibodies Against a Cell Surface Concanavalin a Binding Glycoprotein by James J. Starling; Charles R. Simrell; Paul A. Klein; Kenneth D. Noonan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Production of Monoclonal Antibodies Against a Cell Surface Concanavalin a Binding Glycoprotein about?

Concanavalin A-binding (Con A)-binding cell surface glycoproteins were isolated, via Con A-affinity chromatography, from Triton X-1 00-solubilized Chinese hamster ovary (CHO) cell plasma membranes. The Con A binding glycoproteins isolated in this manner displayed a significantly different profile on sodium dodecyl sulfate-polyacrylamide gels than did the Tritonsoluble surface components, which were not retarded by the Con A-Sepharose column. [ '2sI]-Con A overlays of the pooled column fractions displayed on sodium dodecyl sulfate-polyacrylamide gel electro-phoresis (SDS-PAGE) demonstrated that there were virtually no Con A receptors associated with the unretarded peak released by the Con A-Sepharose column, whereas the material which was bound and specifically eluted from the Con A-Sepharose column with the sugar hapten a-methyl-D-mannopyranoside contained at least 15 prominent bands which bound ['\*'I] -Con A. A receptors, Balb/c mice were immunized with the pooled Con A receptor fraction. Following immunization spleens were excised from the animals and single spleen cell suspensions were fused with mouse myeloma P3/X63-Ag8 cells. Numerous hybridoma clones were subsequently picked

Who reads Production of Monoclonal Antibodies Against a Cell Surface Concanavalin a Binding Glycoprotein?

Author

James J. Starling; Charles R. Simrell; Paul A. Klein; Kenneth D. Noonan

Publisher

Wiley (John Wiley & Sons); Alan R. Liss, Inc.; Wiley (ISSN 0091-7419)

Published

1978

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)