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Neutral sphingomyelinase-2, acid sphingomyelinase, and ceramide levels in COPD patients compared to controls by Simon Lea; Hannah Metcalfe; Jonathan Plumb; Christian Beerli; Chris Poll; Dave Singh; Katharine Abbott-Banner is a Medicine article available to read on EtoBox.

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## Background Increased pulmonary ceramide levels are suggested to play a causative role in lung diseases including COPD. Neutral sphingomyelinase-2 (nSMase-2) and acid SMase (aSMase), which hydrolyze sphingomyelin to produce ceramide, are activated by a range of cellular stresses, including inflammatory cytokines and pathogens, but notably cigarette smoke appears to only activate nSMase-2. Our primary objective was to investigate nSMase-2 and aSMase protein localization and quantification in lung tissue from nonsmokers (NS), smokers (S), and COPD patients. In addition, various ceramide species (C16, C18, and C20) were measured in alveolar macrophages from COPD patients versus controls. ## Materials and methods Patients undergoing surgical resection for suspected or confirmed lung cancer were recruited, and nSMase-2 and aSMase protein was investigated in different areas of lung tissue (small airways, alveolar walls, subepithelium, and alveolar macrophages) by immunohistochemistry. Ceramide species were measured in alveolar macrophages from COPD patients and controls by mass spectrometry. ## Results nSMase-2 and aSMase were detected in the majority of small airways. There was a sign

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Author

Simon Lea; Hannah Metcalfe; Jonathan Plumb; Christian Beerli; Chris Poll; Dave Singh; Katharine Abbott-Banner

Published

2016

Language

EN

Field

Medicine (Health Sciences)