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Maize embryo germination: I. Cell cycle analysis by Elena I. Georgieva; Gerardo López-Rodas; A. Hittmair; H. Feichtinger; G. Brosch; Peter Loidl is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Maize embryo germination: I. Cell cycle analysis about?

The cell-cycle progression of germinating embryos of maize (Zea mays L.) was studied from 0 to 72 h after the start of imbibition using DNA flow cytometry on isolated nuclei, and analyses of thymidine kinase activity, histone biosynthesis and levels of proliferating cell nulcear antigen (PCNA). At the start of germination, 75% of the cells were in G1, but this population had decreased to 25% by 72 h. The concomitant increase of cells in S-phase did not occur continuously, but stepwise, indicating that during germination most of the cells enter S-phase as a partially synchronized population. Within the initial 60 h of embryo germination the cells passed through one S-phase; the start and duration of this period of replicative DNA synthesis was further substantiated by the analysis of S-phase-associated events, the biosynthesis of core histones and the enzyme activity of thymidine kinase, which both began to increase at about 12 h after the start of differentiation. Thymidine kinase fluctuated periodically during germination with a transient maximum at 30 h and a second peak at 72 h; histone biosynthesis was not detectable until 12 h after the start of germination. The levels of PCNA

Who reads Maize embryo germination: I. Cell cycle analysis?

Author

Elena I. Georgieva; Gerardo López-Rodas; A. Hittmair; H. Feichtinger; G. Brosch; Peter Loidl

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)