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HPLC–ICP-MS speciation of selenium in enriched onion leaves – a potential dietary source of Se-methylselenocysteine by Katarzyna Wróbel; Kazimierz Wróbel; Sasi S. Kannamkumarath; Joseph A. Caruso; Irena A. Wysocka; Ewa Bulska; Justyna Świa̧tek; Małgorzata Wierzbicka is a Agricultural and Biological Sciences article available to read on EtoBox.

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In this work chromatographic separation was coupled with mass spectrometry detection to study the speciation of selenium in selenium enriched onion leaves. The plants of onion (Allium cepa L.) were grown hydroponically for one week in standard medium containing inorganic selenium. The leaves and bulbs were separated from roots, the two types of material (leaves and bulbs) were dried and homogenized. The sodium hydroxide (0.1 mol l À1 ) extracts were analyzed by size exclusion chromatography (with spectrophotometric and mass spectrometric detection) showing the incorporation of selenium to the high molecular weight fraction, which was more pronounced in the leaves relative to the bulbs. This incorporation was better with Se(IV) enrichment (33% in leaves and 26% in bulbs) than with Se(VI) (3% and 5%, respectively). After extraction of the low molecular weight compounds (chloroform-methanol-water or 0.4 M perchloric acid-ethanol) and elimination of inorganic selenium (Dowex 1X8), selenium speciation was carried out by ion-pairing high performance chromatography [5 mmol l À1 citric acid, 5 mmol l À1 hexanesulfonic acid, pH 4.5: methanol (95:5)]. The primary organic selenium species fou

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Author

Katarzyna Wróbel; Kazimierz Wróbel; Sasi S. Kannamkumarath; Joseph A. Caruso; Irena A. Wysocka; Ewa Bulska; Justyna Świa̧tek; Małgorzata Wierzbicka

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)

Published

2004

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)