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NMR spectroscopy and MRI investigation of a potential bioartificial liver by Jeffrey M. Macdonald; Mark Grillo; Olga Schmidlin; Dennis T. Tajiri; Thomas L. James is a Medicine article available to read on EtoBox.

What is NMR spectroscopy and MRI investigation of a potential bioartificial liver about?

NMR feasibility was established for a coaxial hydrophobic-membrane bioreactor containing isolated rat hepatocytes with features designed to mimic the human liver. A novel triple-tuned NMR probe and a perfusion system controlling temperature, gas concentrations, flow-rate, and pH were used. We determined the optimum coaxial interfiber distance (i.e. diffusion distance) for maintaining hepatocyte viability in two bioreactor prototypes. Prototype no. 1 and no. 2 had diffusion distances of 500 mm and 200 mm, respectively. Cell viability was established by 31 P NMR and trypan blue exclusion. Only prototype no. 2 maintained cell viability for more than 6 h, indicating the importance of diffusion distance. 31 P spectra obtained over this 6 h time period were similar to in vivo spectra of rat liver. The 31 P spectra were found to be more sensitive to subacute cell viability than trypan blue exclusion. In the 1 H and 31 P spectra, 1 H 2 O and inorganic phosphate signals were split in two at all flow-rates, probably due to bulk magnetic susceptibility effects originating from the three bioreactor compartments. MRI was useful for quality control and determining flow dynamics, fiber integrity,

Who reads NMR spectroscopy and MRI investigation of a potential bioartificial liver?

Author

Jeffrey M. Macdonald; Mark Grillo; Olga Schmidlin; Dennis T. Tajiri; Thomas L. James

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3480)

Published

1998

Language

EN

Field

Medicine (Health Sciences)