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Optimization of Osmotic Blood-brain Barrier Opening to Enable Intravital Microscopy Studies on Drug Delivery in Mouse Cortex by Chengyan Chu; Anna Jablonska; Wojciech G. Lesniak; Aline M. Thomas; Xiaoyan Lan; Raleigh M. Linville; Shen Li; Peter C. Searson; Guanshu Liu; Monica Pearl; Martin G. Pomper; Miroslaw Janowski; Tim Magnus; Piotr Walczak is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Optimization of Osmotic Blood-brain Barrier Opening to Enable Intravital Microscopy Studies on Drug Delivery in Mouse Cortex about?
Intra-arterial (IA) infusion of mannitol induces osmotic blood-brain barrier opening (OBBBO) and that method has been used for decades to improve drug delivery to the brain. However, high variability of outcomes prevented vast clinical adoption. Studies on dynamic multi-scale imaging of OBBBO as well as extravasation of IA injected therapeutic agents are essential to develop strategies assuring precision and reproducibility of drug delivery. Intravital microscopy is increasingly used to capture the dynamics of biological processes at the molecular level in convenient mouse models. However, until now OBBBO has been achieved safely in subcortical structures, which prevented direct insight into the process of extravasation through the skull window. Here, we used our previously developed real-time MRI to adjust the procedure to achieve robust cortical OBBBO. We found that catheter-mediated delivery to the cortex from the ipsilateral carotid artery can be improved by temporarily occluding the contralateral carotid artery. The reproducibility and safety of the method were validated by MRI and histology. This experimental platform was further exploited for studying with intravital microsc
Who reads Optimization of Osmotic Blood-brain Barrier Opening to Enable Intravital Microscopy Studies on Drug Delivery in Mouse Cortex?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Chengyan Chu; Anna Jablonska; Wojciech G. Lesniak; Aline M. Thomas; Xiaoyan Lan; Raleigh M. Linville; Shen Li; Peter C. Searson; Guanshu Liu; Monica Pearl; Martin G. Pomper; Miroslaw Janowski; Tim Magnus; Piotr Walczak
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-3659)
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)