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Thin-layer chromatographic separation of 14C-labelled succinyldicholine, succinylmonocholine and choline by Dharam P. Agarwal; H.Werner Goedde is a Chemistry article available to read on EtoBox.

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The application of the muscIe reiaxant succinyIdicholine (suxamethonium) in anaesthesiology is widespread. In the human body, this compound is mainly hydrolyzed by serum choiinesterase (EC 3.X.1.8), and individuals with a genetically determined atypical form of this enzyme sulTer from prolonged apnoea if they are given succinyidichohne during surgical 1 operations -6\_ The usual diagnostic test for this condition is based on the resistance of the variant (atypical) enzyme to inhibition with dibucaine'. While the "normal" enzyme is inhibited by more than 70%, heterozygotes and homozygotes with the "atypical" alfele show inhibitions of 40-65 % and less than 20x, respectively. Although most of the atypical homozygotes arc predisposed to profonged apnoea after treatment with succinyldicholine, not a!1 of the apnoea cases encountered in clinical practice always show the variant nature of the serum enzyme. Nearly 30 % of patients with prolonged apnoea episodes could not be classified as possessing known variants on the basis of dibucaine inhibition\*-lo. Since the substrate used in this test is usually benzoyIchoIine, no information is available on ahe abiiity of these sera to hydrolyze

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Author

Dharam P. Agarwal; H.Werner Goedde

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1976

Language

EN

Field

Chemistry (Physical Sciences)