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COLD-PCR vs Conventional PCR for BRAF V600E by yanghsitsai is a document available to read on EtoBox.
The correspondence discusses the sensitivity of COLD-PCR combined with HRM for detecting the BRAF V600E mutation compared to conventional methods. The authors express skepticism about the claims of improved sensitivity, citing their own findings that show no enhancement in detecting low-level mutations. They request clarification on discrepancies in melting behavior observed in the study by Mancini et al. and emphasize the need for more rigorous validation of COLD-PCR
- Author
- yanghsitsai
- Language
- EN