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Can I read Trkb Receptors Modulation of Glutamate Release Is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus on EtoBox?
Trkb Receptors Modulation of Glutamate Release Is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus by Daniela B. Pereira; Nelson Rebola; Ricardo J. Rodrigues; Rodrigo A. Cunha; Arsélio P. Carvalho; Carlos B. Duarte is a Neuroscience article available to read on EtoBox.
What is Trkb Receptors Modulation of Glutamate Release Is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus about?
## Abstract Brain‐derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K^+^‐evoked release of [^3^H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [^3^H]glutamate release correlated with a modest increase in phospholipase Cγ (PLCγ) phosphorylation, and with the lack of effect of BDNF on extracellular‐signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one‐third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one‐fourth of total full‐length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density.
Who reads Trkb Receptors Modulation of Glutamate Release Is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Daniela B. Pereira; Nelson Rebola; Ricardo J. Rodrigues; Rodrigo A. Cunha; Arsélio P. Carvalho; Carlos B. Duarte
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)
- Published
- 2006
- Language
- EN
- Field
- Neuroscience (Life Sciences)