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Resistance of Leishmania (Viannia) braziliensis to nitric oxide: correlation with antimony therapy and TNF-α production by Anselmo S Souza; Angela Giudice; Júlia MB Pereira; Luís H Guimarães; Amelia R de Jesus; Tatiana R de Moura; Mary E Wilson; Edgar M Carvalho; Roque P Almeida is a Medicine article available to read on EtoBox.

What is Resistance of Leishmania (Viannia) braziliensis to nitric oxide: correlation with antimony therapy and TNF-α production about?

## Abstract ## Background Nitric oxide (NO) produced in macrophages plays a pivotal role as a leishmanicidal agent. A previous study has demonstrated that 20% of the__L. (V.) braziliensis__isolated from initial cutaneous lesions of patients from the endemic area of Corte de Pedra, Bahia, Brazil, were NO resistant. Additionally, 5 to 11% of the patients did not respond to three or more antimony treatments" (refractory patients). The aim of this study is to investigate if there is an association between the resistance of__L. (V.) braziliensis__to NO and nonresponsiveness to antimony therapy and cytokine production. ## Methods We evaluated the__in vitro__toxicity of NO against the promastigotes stages of__L. (V.) braziliensis__isolated from responsive and refractory patients, and the infectivity of the amastigote forms of these isolates against human macrophages. The supernatants from__Leishmania__infected macrophage were used to measure TNF-α and IL-10 levels. ## Results Using NaNO~2~(pH 5.0) as the NO source,__L. (V.) braziliensis__isolated from refractory patients were more NO resistant (IC50 = 5.8 ± 4.8) than__L. (V.) braziliensis__isolated from responsive patients (IC50 = 2.0 ± 1

Who reads Resistance of Leishmania (Viannia) braziliensis to nitric oxide: correlation with antimony therapy and TNF-α production?

Author

Anselmo S Souza; Angela Giudice; Júlia MB Pereira; Luís H Guimarães; Amelia R de Jesus; Tatiana R de Moura; Mary E Wilson; Edgar M Carvalho; Roque P Almeida

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2334)

Published

2010

Language

EN

Field

Medicine (Health Sciences)