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The assembly and migration of SeqA–Gfp fusion in living cells of Escherichia coli by Toshinari Onogi; Hironori Niki; Mitsuyoshi Yamazoe; Sota Hiraga is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The assembly and migration of SeqA–Gfp fusion in living cells of Escherichia coli about?

SeqA protein, which binds to hemi‐methylated GATC sequences of DNA, is localized to discrete fluorescent foci in wild‐type __Escherichia coli__ cells. In this work, we observed cellular localization of the SeqA–Gfp fusion in living cells. SeqA–Gfp was localized to a discrete focus or foci in wild‐type and __seqA__ null mutant cells, but the fusion was dispersed in the whole cell in __dam__ null mutant cells lacking Dam methyltransferase. These results were consistent with the previous description of the localization of SeqA by immunofluorescence microscopy. Time‐lapse experiments revealed that duplicated SeqA–Gfp foci migrated rapidly in opposite directions. Flow cytometry demonstrated that the fusion restored synchronous replication of chromosomal DNA from multiple origins in __seqA__ null mutant cells, indicating that SeqA–Gfp is biologically active. Immunoprecipitation of the fusion from cell extracts using anti‐Gfp antibody indicated that the fusion was assembled with the wild‐type SeqA protein.

Who reads The assembly and migration of SeqA–Gfp fusion in living cells of Escherichia coli?

Author

Toshinari Onogi; Hironori Niki; Mitsuyoshi Yamazoe; Sota Hiraga

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)