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Enrichment and Characterization of Mouse Putative Epidermal Stem Cells by Jia-xi Zhou; Li-wei Jia; Yong-jun Yang; Sha Peng; Yu-jing Cao; En-kui Duan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Enrichment and Characterization of Mouse Putative Epidermal Stem Cells about?

## Abstract Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal stem cells and culturing them has been challenging. In this study, putative epidermal stem cells of mouse were isolated by combining Hoechst 33342 and propidium iodide staining with fluorescence‐activated cell sorting. Molecular markers expression pattern analysis showed that cytokeratin 14, integrin β~1~ and p63 are expressed in the sorted putative stem cells, but not active β‐catenin, nestin and involucrin. Our results provide further supporting data that mouse putative epidermal stem cells could be successfully isolated by combining Hoechst dye staining with fluorescence‐activated cell sorting and cultured in vitro. The cultured mouse putative epidermal stem cells could be used as a potent tool for studying stem cell biology and testing stem cell therapy.

Who reads Enrichment and Characterization of Mouse Putative Epidermal Stem Cells?

Author

Jia-xi Zhou; Li-wei Jia; Yong-jun Yang; Sha Peng; Yu-jing Cao; En-kui Duan

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)