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Can I read Retention Behaviour of a Bonded Reversed Phase in a High-performance Liquid Chromatographic Assay of Serum Theophylline on EtoBox?
Retention Behaviour of a Bonded Reversed Phase in a High-performance Liquid Chromatographic Assay of Serum Theophylline by R.E. Hill is a Chemistry article available to read on EtoBox.
What is Retention Behaviour of a Bonded Reversed Phase in a High-performance Liquid Chromatographic Assay of Serum Theophylline about?
A simple, rapid assay for serum theophylline is described. Optimisation of the high-performance liquid chromatographic system has allowed the analysis of minimum sample volume (50~1) in the shortest time (8 min) with good between-batch precision (C.V. = 8.3 "/,) and accuracy (recovery CLI. 100 %)\_ The behaviour of theophylline and other xanthine derivatives, theobromine, caffeine and %chlorotheophylline, on a bonded octadecyi reversed-phase system suggests that the C,, phase effects solute separation by mixed retention mechanisms rather than by pure reversedphase chromatography. This behaviour is attributed to the participation of underivatised silanol groups in the chromatographic process, an observation corroborated by independent investigation. The mechanism of the silanol participation is difhcult to rationalise; however, a normal-phase liquid partition system complies with the experimental data.
Who reads Retention Behaviour of a Bonded Reversed Phase in a High-performance Liquid Chromatographic Assay of Serum Theophylline?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- R.E. Hill
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1977
- Language
- EN
- Field
- Chemistry (Physical Sciences)