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Expression of Epithelial Calcium Transport System in Rat Cochlea and Vestibular Labyrinth by Daisuke Yamauchi; Kazuhiro Nakaya; Nithya N Raveendran; Donald G Harbidge; Ruchira Singh; Philine Wangemann; Daniel C Marcus is a Medicine article available to read on EtoBox.
What is Expression of Epithelial Calcium Transport System in Rat Cochlea and Vestibular Labyrinth about?
## Abstract ## Background The low luminal Ca^2+^ concentration of mammalian endolymph in the inner ear is required for normal hearing and balance. We recently reported the expression of mRNA for a Ca^2+^-absorptive transport system in primary cultures of semicircular canal duct (SCCD) epithelium. ## Results We now identify this system in native vestibular and cochlear tissues by qRT-PCR, immunoblots and confocal immunolocalization. Transcripts were found and quantified for several isoforms of epithelial calcium channels (TRPV5, TRPV6), calcium buffer proteins (calbindin-D9K, calbindin-D28K), sodium-calcium exchangers (NCX1, NCX2, NCX3) and plasma membrane Ca^2+^-ATPase (PMCA1, PMCA2, PMCA3, and PMCA4) in native SCCD, cochlear lateral wall (LW) and stria vascularis (SV) of adult rat as well as Ca^2+^ channels in neonatal SCCD. All components were expressed except TRPV6 in SV and PMCA2 in SCCD. 1,25-(OH)~2~vitamin D~3~ (VitD) significantly up-regulated transcripts of TRPV5 in SCCD, calbindin-D9K in SCCD and LW, NCX2 in LW, while PMCA4 in SCCD and PMCA3 in LW were down-regulated. The expression of TRPV5 relative to TRPV6 was in the sequence SV > Neonatal SCCD > Adult SCCD > LW > prima
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- Author
- Daisuke Yamauchi; Kazuhiro Nakaya; Nithya N Raveendran; Donald G Harbidge; Ruchira Singh; Philine Wangemann; Daniel C Marcus
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6793)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Life Sciences)