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Method for the analysis of the methylphosphonic acid metabolites of sarin and its ethanol-substituted analogue in urine as applied to the victims of the Tokyo sarin disaster by Masayasu Minami; Da-Mei Hui; Masao Katsumata; Hirofumi Inagaki; Camille A Boulet is a Chemistry article available to read on EtoBox.

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An analysis method for the methylphosphonic acid metabolites of sarin in urine using trimethylsilyl derivatization and flame photometric detection is described in this report. Authentic reference standards of isopropyl methylphosphonic acid (IMPA) and ethyl methylphosphonic acid (EMPA) as well as methylphosphonic acid were employed to estimate the concentration in human urine. A sample pretreatment procedure was developed for urine using a column of cation-loaded ion-exchange resins (Ag -, Ba -or H -Dowex) and adjusting the pH of the eluate from the column to 3.75-3.85 improved recovery of the target compounds. The eluate was evaporated to dryness under vacuum prior to trimethylsilylation, to remove water and any hydroxy-or amino-carrying volatile substances. The sarin metabolites, because of their low volatility, were concentrated and could be derivatized for analysis. The use of synthesized authentic sarin and ethylsarin metabolites, i.e., IMPA and EMPA, made it possible to establish the necessary sample pretreatment procedures for derivatization and gas chromatography-flame photometric detection (GC-FPD) analysis. The detection limits were 0.025 ppm both for EMPA and IMPA, and 0

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Author

Masayasu Minami; Da-Mei Hui; Masao Katsumata; Hirofumi Inagaki; Camille A Boulet

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1997

Language

EN

Field

Chemistry (Physical Sciences)