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What is In Vivo CRISPR Screening with SCAR Vectors about?

This document outlines a protocol for in vivo CRISPR screening using selective CRISPR antigen removal (SCAR) lentiviral vectors, aimed at overcoming limitations posed by immune recognition of Cas9 and other proteins. The protocol is designed for use in a Renca mouse model and provides detailed steps for conducting genetic screens with sgRNA libraries across various cell lines. It emphasizes the importance of optimizing lentivirus preparation, transduction rates, and engraftment rates to ensure effective exp

Author

xevomob

Language

EN